• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Fu, Zhangcheng (Fu, Zhangcheng.) [1] | Lv, Tong (Lv, Tong.) [2] | Jiang, Lili (Jiang, Lili.) [3] | Xing, Chao (Xing, Chao.) [4] | Lu, Chunhua (Lu, Chunhua.) [5] (Scholars:卢春华)

Indexed by:

EI SCIE

Abstract:

Selective and sensitive detection of microRNA is crucial for early diagnosis and pathogenesis of disease. Here, we established a novel electrochemical biosensor for simple and accurate analysis of the tumor biomarker microRNA-141, which was based on in-situ catalytic hairpin assembly (CHA) actuated DNA tetrahedral (DTN) interfacial probes. Two hairpin structures used for CHA reaction were placed on the DTN, in which the hairpin H1 on the one vertex of DTN and hairpin H2 embedded in adjacent edge, respective. The target microRNA-141 could open the hairpin H1 and activated the in-situ CHA reaction between H1 and H2 to alter the conformational of DTN, increasing the chances of the direct interaction between methylene blue (MB) and the electrode surface, leading to an increase in the electrochemical signal. Meanwhile, the released miRNA-141 could unfold another H1, enabling the enzyme-free recycling of the target to obtain amplified electrochemical signals. Moreover, the in-situ catalytic hairpin assembly reaction on DTN could shorten the reaction time and enhance the sensitivity. The established biosensor exhibited a wide linear dynamic range of miRNA-141 from 1 fM to 100 pM with a detection limit of 0.32 fM. Besides, the approach can discriminate the target miRNA from mismatched ones with excellent selectivity and can be successfully applied in diluted serum samples, holding great potential for sensitive detection of various biomarkers clinically.

Keyword:

Catalytic hairpin assembly DNA tetrahedral Nanostructure Electrochemical In-situ miRNA detection

Community:

  • [ 1 ] [Fu, Zhangcheng]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol,State K, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 2 ] [Lv, Tong]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol,State K, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 3 ] [Jiang, Lili]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol,State K, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 4 ] [Lu, Chunhua]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol,State K, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 5 ] [Xing, Chao]Minjiang Univ, Ctr Adv Marine Mat & Smart Sensors, Fujian Key Lab Funct Marine Sensing Mat, Fuzhou 350108, Peoples R China

Reprint 's Address:

  • 卢春华

    [Lu, Chunhua]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol,State K, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China;;[Xing, Chao]Minjiang Univ, Ctr Adv Marine Mat & Smart Sensors, Fujian Key Lab Funct Marine Sensing Mat, Fuzhou 350108, Peoples R China

Show more details

Related Keywords:

Source :

TALANTA

ISSN: 0039-9140

Year: 2021

Volume: 233

6 . 5 5 6

JCR@2021

5 . 6 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:117

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count: 16

SCOPUS Cited Count: 13

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

Online/Total:159/10019404
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1