Plants　have　astonishing　ability　to　yield　and　release　a　variety　of　organic　and　inorganic　compounds　into　the　rhizosphere　known　as　root　exudates.　The　normal　yield　of　these　root　exudates　can　be　enhanced　by　applying　various　abiotic　stresses.　In　current　research　abiotic　stresses　i.e.　physical　and　chemical　have　been　used　to　get　desired　outcomes　for　root　exudates　of　canola　plant.　Effect　of　abiotic　stress　on　total　phenolic　content　and　antioxidant　activity　of　root　exudates　has　also　been　observed.　The　results　showed　that　by　applying　abiotic　stress　the　obtained　yield　came　out　in　the　range　1.56　±　1.79　to　1.97　±　1.74　percent/100　g　DW　which　was　0.03　percent/100g　DW　without　applying　abiotic　stress.　In　addition　to　this　TPC　results　ranged　31.92　±　2.22　-　511.3　±　10.27　mg　GAE/g　of　DW　that　was　higher　as　compared　to　the　value　of　control　(11.2　±　2.11　mg　GAE/g　of　DW).　The　value　of　IC50　calculated　by　the　DPPH　radical　scavenging　assay　was　about　0.58　±　0.02　-　1.71　±　0.03　mg/mL　with　abiotic　stress　as　compared　to　the　value　of　control　to　be　2.1　±　0.03　mg/mL　that　indicated　the　increasing　antioxidant　activity　with　respect　to　abiotic　stress　application.　©　2021　SPIE.