Ru(dcbpy)32+-polyethyleneimine-L-lysine　(Ru-PEI-L-lys)　had　been　immobilized　on　metal　organic　frameworks　(ZIF-8)　to　form　an　electrochemiluminescent(ECL)　indicator　(Ru-PEI-L-lys-ZIF-8).　In　this　ECL　indicator,　PEI-L-lys　is　used　as　a　co-reactant.　Platinum　nanoparticles　(PtNPs)　has　been　mixed　with　Ru-PEI-L-lys-ZIF-8　to　form　a　thin　film　to　increase　the　electron　transfer　rate　and　enhanced　the　ECL　response　of　the　system.　The　prepared　material　had　been　characterized　carefully　and　been　combined　with　high　selectivity　of　aptamer　to　develop　a　ECL　biosensor　for　thrombin　detection.　RecJf　exonuclease　(an　ssDNA　specific　exonuclease)　assistant　target　recycling　amplification　has　been　adopted　to　enhance　the　sensitivity　of　the　system.　The　ECL　response　of　the　system　has　a　linear　relationship　with　logarithm　of　thrombin　concentration　in　the　range　of　1　fM　to　10　pM　with　a　detection　limit　of　0.02　aM.　This　work　not　only　provides　a　new　strategy　for　the　design　and　synthesis　of　high　performance　and　stable　ECL　indicator,　but　also　opens　up　a　new　approach　for　the　development　of　highly　sensitive　ECL　sensors　for　biological　analysis.　©　2021　Elsevier　B.V.