An　ultra-high　performance　liquid　chromatography　tandem　mass　spectrometry　(UPLC-MS/MS)　method　was　established　and　validated　for　the　simultaneous　determination　of　six　glycosidic　aroma　precursors　in　pomelo,　including　geraniol　β-glucoside,　geraniol　β-primeveroside,　linalool　β-primeveroside,　benzyl　β-primeveroside,　2-phenylethyl　β-primeveroside　and　nerolidol　β-primeveroside.　The　results　showed　that　the　proposed　method　has　the　advantages　of　rapidity,　high　sensitivity,　and　good　accuracy.　Six　glycosidic　aroma　precursors　were　effectively　separated　in　a　short　run　time　(13　min),　and　the　limit　of　detection,　limit　of　quantification,　recovery,　and　repeatability　of　analytes　were　0.321-4.47　ng　mL-1,　1.07-14.9　ng　mL-1,　94.4-109.1%,　and　5.2-14.5%,　respectively.　The　developed　method　was　applied　to　analyze　the　contents　of　glycosidic　aroma　precursors　in　different　organs　of　pomelo　plant,　including　leaves,　flowers　and　fruits.　The　analytical　result　showed　that　glycosidic　aroma　precursor　contents　in　plant　leaves,　flowers　and　fruits　were　in　the　range　of　0-5964.9　μg　kg-1,　and　more　glycosidic　aroma　precursors　were　found　in　flowers　than　in　leaves　or　fruits.　It　is　envisioned　that　the　proposed　UPLC-MS/MS　method　have　promising　application　in　qualifying　and　quantifying　these　six　glycosidic　aroma　precursors　in　pomelo.　©　The　Royal　Society　of　Chemistry.