A　facile　and　rapid　strategy　for　preparation　of　covalent　organic　framework　(COF)　coated　fibers　at　ambient　temperature　is　urgently　needed　for　solid-phase　microextraction　(SPME)　technology.　In　this　work,　an　in　situ　room-temperature　rapid　growth　strategy　was　developed　to　high-efficiently　fabricate　imine-linked　COF　(TPB-DVA)　coated　fibers　in　as　little　as　30　min　at　room　temperature,　and　the　thickness　of　the　coating　reached　9　μm.　The　prepared　TPB-DVA　coated　fiber　offer　high　thermal　and　chemical　stability,　and　outstanding　service　lifetime.　Moreover,　we　generalize　this　strategy　to　other　two　imine-linked　COF　(TPB-DMTP　and　TFPB-TAPB)　coated　fibers　and　the　fibers　were　fabricated　at　room　temperature　for　3　h　and　12　h,　respectively,　which　demonstrate　the　applicability　of　this　strategy.　Subsequently,　a　SPME-GC-MS/MS　analytical　method　was　developed　for　trace　pyrethroids　(PYs)　detection,　which　exhibited　high　enhancement　factors　(EFs,　2700–13195),　wide　linear　range　(0.08–800　ng　L−1),　low　limits　of　detection　(LODs,　0.02–0.20　ng　L−1),　and　good　repeatability　(RSD　≤　8.5%,　n　=　6).　Furthermore,　the　developed　analytical　method　was　applied　to　tea　samples　and　trace　PYs　(1.31–4.32　ng　L−1)　were　found　with　satisfactory　recovery　(80.2–119.8%).　The　above　results　demonstrated　that　the　feasibility　of　the　developed　strategy　for　the　facile　and　rapid　fabrication　of　imine-linked　COF　coated　fibers.　©　2021　Elsevier　B.V.