Early　diagnosis　of　cancers　relies　on　the　sensitive　detection　of　specific　biomarkers,　but　most　of　the　current　testing　methods　are　inaccessible　to　home　healthcare　due　to　cumbersome　steps,　prolonged　testing　time,　and　utilization　of　toxic　and　hazardous　substances.　Herein,　we　developed　a　portable　self-powered　photoelectrochemical　(PEC)　sensing　platform　for　rapid　detection　of　prostate-specific　antigen　(PSA,　as　a　model　disease-related　protein)　by　integrating　a　self-powered　photoelectric　signal　output　system　catalyzed　with　chemiluminescence-functionalized　Au　nanoparticles　(AuNPs)　and　a　phosphomolybdic　acid　(PMA)-based　photochromic　visualization　platform.　TiO2-g-C3N4-PMA　photosensitive　materials　were　first　synthesized　and　functionalized　on　a　sensor　chip.　The　sensor　consisted　of　filter　paper　modified　with　a　photocatalytic　material　and　a　regional　laser-etched　FTO　electrode　as　an　alternative　to　a　conventional　PEC　sensor　with　a　glass-based　electrode.　The　targeting　system　involved　a　monoclonal　anti-PSA　capture　antibody-functionalized　Fe3O4magnetic　bead　(mAb1-MB)　and　a　polyclonal　anti-PSA　antibody　(pAb2)-N-(4-aminobutyl)-N-ethylisoluminol-AuNP　(ABEI-AuNP).　Based　on　the　signal　intensity　of　the　chemiluminescent　system,　the　photochromic　device　color　changed　from　light　yellow　to　heteropoly　blue　through　the　PMA　photoelectric　materials　integrated　into　the　electrode　for　visualization　of　the　signal　output.　In　addition,　the　electrical　signal　in　the　PEC　system　was　amplified　by　a　sandwich-type　capacitor　and　readout　on　a　handheld　digital　multimeter.　Under　optimum　conditions,　the　sensor　exhibited　high　sensitivity　relative　to　PSA　in　the　range　of　0.01-50　ng　mL-1with　a　low　detection　limit　of　6.25　pg　mL-1.　The　flow-through　chemiluminescence　reactor　with　a　semiautomatic　injection　device　and　magnetic　separation　was　avoid　of　unstable　light　source　intensity　inherent　in　the　chemiluminescence　process.　Therefore,　our　strategy　provides　a　new　horizon　for　point-of-care　analysis　and　rapid　cost-effective　clinical　diagnosis.　©　2021　American　Chemical　Society