Lactobacillus　paracasei　FZU103,　a　probiotic　previously　isolated　from　the　traditional　brewing　process　of　Hongqu　rice　wine,　may　have　the　beneficial　effect　of　improving　the　disorder　of　lipid　metabolism.　This　study　aimed　to　determine　the　beneficial　effects　of　L.　paracasei　FZU103　on　improving　hepatic　lipid　accumulation　associated　with　hyperlipidemia.　Results　indicated　that　L.　paracasei　FZU103　intervention　significantly　inhibited　the　abnormal　growth　of　body　weight　and　epididymal　white　adipose　tissue　(eWAT),　prevented　the　hypertrophy　of　epididymal　adipocytes,　ameliorated　the　biochemical　parameters　of　serum　and　liver　related　to　lipid　metabolism　in　HFD-fed　mice.　Histological　analysis　also　showed　that　the　excessive　accumulation　of　lipid　droplets　in　the　livers　induced　by　HFD-feeding　was　greatly　alleviated　by　L.　paracasei　FZU103　intervention.　In　addition,　L.　paracasei　FZU103　also　promoted　the　excretion　of　bile　acids　(BAs)　through　feces.　Metagenomic　analysis　revealed　that　oral　supplementation　with　L.　paracasei　FZU103　significantly　increased　the　relative　abundance　of　Ruminococcus,　Alistipes,　Pseudoflavonifractor　and　Helicobacter,　but　decreased　the　levels　of　Blautia,　Staphylococcos　and　Tannerella　in　HFD-fed　mice.　The　relationships　between　lipid　metabolic　parameters　and　intestinal　microbial　phylotypes　were　also　revealed　by　correlation　heatmap　and　network.　Furthermore,　ultra-performance　liquid　chromatography-quadrupole　time-of-flight　mass　spectrometry　(UPLC-QTOF/MS)-based　liver　metabolomics　demonstrated　that　L.　paracasei　FZU103　had　a　significant　regulatory　effect　on　the　metabolic　pathways　of　glycerophospholipid　metabolism,　fatty　acid　degradation,　fatty　acid　elongation,　unsaturated　fatty　acids　biosynthesis,　riboflavin　metabolism,　glycerolipid　metabolism,　primary　bile　acid　biosynthesis,　arachidonic　acid　metabolism,　etc.　Additionally,　L.　paracasei　FZU103　intervention　regulated　expression　of　hepatic　genes　involved　in　lipid　metabolism　and　bile　acid　homeostasis,　and　promoted　fecal　excretion　of　intestinal　BAs.　These　findings　present　new　evidence　supporting　that　L.　paracasei　FZU103　has　the　potential　to　improve　lipid　metabolism,　and　could　be　used　as　a　potential　functional　food　for　the　prevention　of　hyperlipidemia.　©　2020　Elsevier　Ltd