In　this　work,　response　surface　methodology　was　used　to　optimize　the　fermentation　medium　components　of　E.　cristatum　FS-10　producing　antibacterial　active　substances.　Meanwhile,　the　antibacterial　activity　products　were　isolated　and　preliminary　identification　through　highperformance　liquid　chromatography　(HPLC),　matrix-assisted　laser　desorption　ionization　time-of-flight　mass　spectrometry　(MALDI-TOF-MS)　and　Fourier　transform　infrared　(FT-IR)　spectrometry.　The　results　showed　that　the　optimal　medium　components　were　determined　as　follows:　anhydrous　glucose　(carbon　source)　7.5%,　sodium　nitrate　(nitrogen　source)　0.65%,　and　potassium　chloride　4.5%.　Under　the　optimized　fermentation　conditions,　the　bacteriostasis　rate　reached　54.4%　against　the　suspension　of　Psychrophile　bacteria(1×105　CFU/mL).　Two　antibacterial　active　components(FS-IV　and　FS-VI)　were　isolated　and　identified.　The　molecular　mass　of　component　FS-IV　and　FS-VI　were　1　630.8　u　and　840.5　u,　respectively,　which　might　imply　the　existence　of　heteropolysaccharide　derivative　compounds　and　amide　derivative　compounds.　Antibacterial　compounds　could　inhibit　the　growth　of　some　foodborne　bacteria,　including　Pseudomonas,　Shewanella　and　Psychrophile.　They　were　stable　to　heat　and　acid-base　conditions.　©　2022,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.