Sensitive　and　accurate　miRNAs　assay　is　critical　for　early　diagnosis　of　non-small-cell　lung　carcinomas　(NSCLC).　Herein,　we　demonstrate　a　photothermal　and　electrochemical　dual-readout　assay　method　for　miRNA　detection　based　on　a　novel　biocatalysis-mediated　MOF-to-prussian　blue　(PB)　transformation　(BMMPT)　strategy　and　the　catalytic　hairpin　assembly　(CHA)　amplification　strategy.　It　is　found　that　the　Fe2+-based　MOF　(MOF-Fe2+)　can　act　as　the　Fe2+　source　to　react　with　K3[Fe(CN)6],　leading　to　the　in-situ　formation　of　prussian　blue　(PB)　on　MOF-Fe2+.　Due　the　inherent　near-infrared　(NIR)　photothermal　conversion　ability　and　electrochemical　signal　of　PB,　the　resulting　PB@MOF-Fe2+　is　employed　to　arouse　temperature　readout　or　electrochemical　signal.　The　presence　of　target　miRNA-21　triggers　the　CHA　reaction　on　magnetic　beads　(MBs),　resulting　the　capture　of　numerous　glucose　oxidase　(GOx)　tags　on　MBs.　The　GOx　tags　then　catalyze　the　generation　of　H2O2　using　glucose　as　substrate.　The　H2O2　is　used　to　inhibit　the　MOF-to-PB　transformation　process　by　oxidizing　Fe2+　into　Fe3+,　leading　to　the　decrease　in　temperature　and　electrochemical　readout　aroused　by　PB@MOF-Fe2+.　By　this　means,　a　signal-off　assay　mode　with　dual　readout　is　established　for　miRNA-21.　Under　the　optimal　conditions,　using　temperature　readout　or　electrochemical　readout,　miRNA-21　can　be　detected　at　concentrations　as　low　as　0.3　fM　and　0.32　fM,　respectively.　Moreover,　the　developed　method　is　successfully　applied　to　evaluate　the　expression　level　of　miRNA-21　in　serum　of　NSCLC　patients.　This　work　not　only　provides　a　practical　tool　for　NSCLC　diagnosis　but　also　presents　the　new　features　of　MOF　materials　as　signal　transduction　tags.　©　2022　Elsevier　B.V.