Type　2　diabetes　mellitus　(T2DM)　may　lead　to　abnormally　elevated　blood　glucose,　lipid　metabolism　disorder,　and　low-grade　inflammation.　Besides,　the　development　of　T2DM　is　always　accompanied　by　gut　microbiota　dysbiosis　and　metabolic　dysfunction.　In　this　study,　the　T2DM　mice　model　was　established　by　feeding　a　high-fat/sucrose　diet　combined　with　injecting　a　low　dose　of　streptozotocin.　Additionally,　the　effects　of　oral　administration　of　ethanol　extract　from　Sanghuangporous　vaninii　(SVE)　on　T2DM　and　its　complications　(including　hypoglycemia,　hyperlipidemia,　inflammation,　and　gut　microbiota　dysbiosis)　were　investigated.　The　results　showed　SVE　could　improve　body　weight,　glycolipid　metabolism,　and　inflammation-related　parameters.　Besides,　SVE　intervention　effectively　ameliorated　the　diabetes-induced　pancreas　and　jejunum　injury.　Furthermore,　SVE　intervention　significantly　increased　the　relative　abundances　of　Akkermansia,　Dubosiella,　Bacteroides,　and　Parabacteroides,　and　decreased　the　levels　of　Lactobacillus,　Flavonifractor,　Odoribacter,　and　Desulfovibrio　compared　to　the　model　group　(LDA　＞　3.0,　p　＜　0.05).　Metabolic　function　prediction　of　the　intestinal　microbiota　by　PICRUSt　revealed　that　glycerolipid　metabolism,　insulin　signaling　pathway,　PI3K-Akt　signaling　pathway,　and　fatty　acid　degradation　were　enriched　in　the　diabetic　mice　treated　with　SVE.　Moreover,　the　integrative　analysis　indicated　that　the　key　intestinal　microbial　phylotypes　in　response　to　SVE　intervention　were　strongly　correlated　with　glucose　and　lipid　metabolism-associated　biochemical　parameters.　These　findings　demonstrated　that　SVE　has　the　potential　to　alleviate　T2DM　and　its　complications　by　modulating　the　gut　microbiota　imbalance.