In　the　process　of　instrumental　strength　training,　real-Time　monitoring　of　muscle　state　changes　is　of　great　significance.　This　paper　mainly　studies　the　Electrical　Impedance　Myography　(EIM)　multi-frequency　measurement　system　based　on　virtual　instruments　to　track　the　changes　of　muscle　state　in　real-Time.　Firstly,　the　multi-frequency　excitation　signal　is　realized　on　LabVIEW　and　transmitted　by　the　DAQmx　driver　function.　The　signal　is　converted　by　a　constant　current　source　and　output　to　the　human　body.　The　signal　receiver　realizes　signal　acquisition,　window　function　design,　and　demodulation　unit　design.　Finally,　the　time　parameter　processing　method　is　proposed,　and　the　time-varying　impedance　spectrum　changing　synchronously　with　the　muscle　state　is　collected.　©　2022　IEEE.