Shewanella　and　Pseudomonas　are　considered　to　be　the　main　spoilage　bacteria　for　chilled　fish.　This　work　focused　on　the　spoilage　and　adhesion　ability　of　two　spoilage　bacteria　(Shewanella　spp.　S-1　and　Pseudomonas　spp.　P-1),　and　the　adhesion　inhibitory　effects　of　Bacillus　subtilis　against　the　spoilage　bacteria　in　large　yellow　croaker　(Pseudosciaena　crocea).　We　aimed　to　develop　a　feasible　solution　to　prolong　the　shelf　life　of　large　yellow　croaker.　The　spoilage　and　adhesion　characteristics　of　Shewanella　spp.　and　Pseudomonas　spp.　strains　were　compared.　It　was　found　that　Shewanella　spp.　induced　more　significant　spoilage　of　large　yellow　croaker　than　Pseudomonas　spp.　at　low　temperature　in　vitro.　In　addition,　Shewanella　spp.　demonstrated　stronger　adhesion　to　the　intestinal　mucus　than　Pseudomonas　spp.　in　vitro.　The　results　of　a　one-week　feeding　experiment　showed　that　Shewanella　was　more　likely　to　adhere　to　large　yellow　croaker　intestines　than　Pseudomonas.　The　supplementation　of　B.　subtilis　strain　BS08　effectively　inhibited　the　proliferation　of　Shewanella　and　slowed　the　spoilage　process　of　large　yellow　croaker.