The　aim　of　this　study　was　to　investigate　the　protective　effect　and　mechanism　of　bigeye　tuna　(Thunnus　obesus)　skin　collagen　peptides　(TSCP)　and　bone　collagen　peptides　(TBCP)　on　skin　photoaging.　In　UVB-damaged　NIH/3T3　cells,　TSCP　and　TBCP　could　effectively　scavenge　excessive　reactive　oxygen　species,　suppress　mRNA　and　protein　expression　of　MMP-1,　and　inhibit　the　decreased　mRNA　and　protein　expression　of　TGF-β1　and　type　I　collagen.　In　the　ICR　mice　model　exposed　to　UVB,　TSCP　and　TBCP　could　effectively　improve　moisture　content,　Hyp　content　and　total　antioxidant　capacity　level,　activate　TGF-β1　signaling　pathway,　and　inhibit　MAPK　signaling　pathway,　thereby　retarding　UVB-induced　skin　photoaging.　TSCP　exhibited　more　excellent　antioxidant　activity,　inhibited　p-p38　activation　and　promoted　TGF-β1　expression　via　TGF-β　signaling　pathway.　By　contrast,　TBCP　was　more　potent　to　down-regulate　protein　expression　of　p-ERK,　p-JNK　and　MMP-1.　The　present　work　provides　a　theoretical　basis　for　employment　of　collagen　peptides　to　attenuate　UVB-induced　photoaging.　©　2022