Functionalized　two-dimensional　Ti3C2Tx　(TN-EHL)　was　prepared　as　an　effective　adsorbent　for　removal　of　methylene　blue　dye　(MB)　and　copper　ions　(Cu2+).　Enzymatic　hydrolysis　lignin　(EHL),　a　reproducible　natural　resource,　was　used　to　functionalize　the　Ti3C2Tx　nanosheets.　EHL　can　not　only　introduce　active　functional　groups　into　TN-EHL　but　also　prevent　the　oxidation　of　Ti3C2Tx,　thus　promoting　the　adsorption　performance　of　TN-EHL.　The　maximum　adsorption　capacities　of　TN-EHL50　(in　which　the　EHL　content　is　50　wt%)　for　MB　and　Cu2+　were　293.7　mg　g−1　and　49.96　mg　g−1,　respectively.　The　higher　correlation　coefficients　(R2)　of　MB　(0.9996)　and　Cu2+　(0.9995)　indicating　that　their　adsorption　processes　can　be　described　by　the　pseudo-second-order　kinetic　model.　The　MB　adsorption　data　fit　the　Freundlich　isotherm　with　R2　of　0.9953,　whereas　the　Cu2+　ions　adsorption　data　fit　the　Langmuir　isotherm　with　R2　of　0.9998.　The　thermodynamic　analysis　indicates　that　the　adsorption　process　of　MB　and　Cu2+　on　TN-EHL50　is　spontaneous　and　endothermic.　Significantly,　the　Cu2+　ions　were　reduced　to　Cu2O　and　CuO　particles　during　the　adsorption　process.　Therefore,　TN-EHL　has　a　great　potential　as　an　environmentally　friendly　adsorbent　for　MB　removal　and　recovery　of　Cu2+　ions　from　wastewater.　©　2022　Elsevier　B.V.