A　novel　molecularly　imprinted　monolithic　(MIM)　column　was　designed　and　fabricated　using　the　epitope　approach,　and　was　used　for　the　selective　capillary　microextraction　(CME)　of　the　neuropeptides　neurotensin　(NT)　and　neuromedin　N　(NmN).　The　MIMs　were　synthesized　in　a　capillary　by　thermally　initiated　polymerization　of　the　functional　monomer　(methacrylic　acid　(MAA)),　in　the　presence　of　a　dummy　template　(Pro-Tyr-Ile-Leu　(PYIL)),　a　crosslinker　and　porogens.　The　resulting　monoliths　were　characterized　by　scanning　electron　microscopy,　pore　size　distribution　measurement,　and　Fourier　transform　infrared　spectroscopy.　Different　synthesis　conditions　of　the　MIM　column　were　investigated.　The　parameters　affecting　the　MIM-CME　performance,　including　loading,　washing　and　elution　protocols,　were　optimized　as　well.　The　MIMs　were　used　to　enrich　NT　and　NmN　from　human　plasma　prior　to　HPLC-UV　analysis.　The　imprinted　monolith　showed　an　excellent　maximum　adsorption　capacity　of　245–711　mg　mL−1　and　selectivity　(imprinting　factor　of　5.7–13.4)　towards　its　target　peptides.　Low　detection　limits　of　0.62　and　1.20　nM,　and　satisfactory　recoveries　(82.5–98.8%)　were　obtained　for　NT　and　NmN,　respectively.　The　proposed　MIM-CME/HPLC-UV　method　was　found　suitable　to　be　used　as　an　effective　tool　for　the　highly　efficient　and　specific　analysis　of　NT　and　NmN　in　human　plasma　samples.　©　2022　Elsevier　B.V.