Currently,　this　has　been　a　great　challenge　working　in　the　field　of　fabricating　flexible　pressure　sensors　with　high　sensitivity,　wide　linearity,　and　a　simple　manufacturing　process　for　practical　applications.　Meanwhile,　flexible　piezoresistive　sensors　have　caused　considerable　interest　in　the　electronic　skin　and　healthcare　detection　systems　for　sensing　biophysical　signals　such　as　pulse　waves,　respiration,　and　intraocular　pressure.　Reading　hidden　health　information　by　imitating　the　perceptual　behavior　of　organisms　has　become　a　very　active　developed　area　of　research.　However,　the　lack　of　effective　signal　transduction　is　a　notable　important　limitation　of　the　study　for　sensors　to　detect　biochemical　signals,　such　as　metabolites,　proteins,　which　contain　more　reference　value　and　health　information.　Therefore,　we　firstly　prepared　a　multiscale　hierarchical　designed　pressure　sensor　based　on　polyaniline　urchin-like　hollow　spheres　(PANI-UHSs)　as　conductive　material　mixed　thermoplastic　polyurethane　(TPU)　substrate,　which　exhibited　a　satisfactory　sensitivity　of　17.8　kPa−1　in　the　pressure　range　of　0–160　kPa.　In　this　work,　a　simple　way　to　address　the　issue　was　proposed　using　the　sensor　combined　with　a　gas-producing　immunoassay　reaction,　resulting　in　a　broad　and　sensitive　determination　of　the　target　carcinoembryonic　antigen　(CEA).　Furthermore,　it　would　be　also　interesting　to　the　design　of　array-based　immunoassay　device　which　conceptually　validates　the　application　potential　of　flexible　sensors　in　high-throughput　biomedical　detection.　It　is　expected　that　the　introduction　of　this　strategy　will　become　a　paradigm　that　inspires　broader　applications　of　flexible　sensors　for　more　comprehensive　medical　detection,　molecular　diagnostics,　and　faster　portable　biomedical　analysis.　©　2022　Elsevier　Ltd