An　electrochemical　sensor　based　on　loading　molecularly　imprinted　polymers　(MIP)　on　the　material　surface　can　improve　the　specificity　towards　the　object.　In　this　work,　a　T-shaped　PbTiO3　with　a　high　active-exposed　(110)　facet　was　prepared　by　a　hydrothermal　process.　Then,　Ag　nanoparticles　(Ag　NPs)　modified　T-shaped　PbTiO3　was　obtained　by　in-situ　photocatalytic　reduced　method　under　UV　irradiation,　where　a　hetero-junction　was　formed　with　a　well　lattice　matching　between　the　(111)　facet　of　Ag0　and　the　(110)　facet　of　PbTiO3.　A　MIPs　modified　by　Ag　nanoparticles　(NPs)/PbTiO3　(MAP)　electrodes　was　prepared　via　electro　polymerization　process　by　o-Phenylenediamine　(o-PD)　in　the　presence　of　the　template　molecule,　bovine　hemoglobin　(BHb),　i.e.,　the　detected　molecule.　The　response　peak　current　and　concentration　of　BHb　is　demonstrated　with　a　good　linear　relationship　in　the　range　of　0.00294–0.41　nM　(R2　=0.98),　and　the　detection　limit　at　0.23　pM　(S/N　=　3).　A　heterojunction　between　Ag　NPs　and　high-　active　facet　of　PbTiO3　is　beneficial　to　oxidizing　electroactive　material　([Fe　(CN)6]3-/4-),　generating　more　BHb-imprinting　cavities　on　the　modified　electrode　and　improving　the　sensitivity　of　sensor.　The　electrochemical　sensor　is　with　a　simple,　stable　structure　and　high　sensitivity　to　BHb　detection.　Furthermore,　the　sensor　was　successfully　applied　to　detect　BHb　in　the　bovine　serum　samples.　©　2022　Elsevier　B.V.