Frozen　storage　of　3D　printing　ink　is　an　inevitable　trend,　but　it　may　impair　the　processing　properties　of　surimi　ink.　Antifreeze　peptides　(AFPs)　show　potential　application　foreground　in　3D　printing.　In　this　study,　the　rheological　properties　in　3D　printing　extrusion,　recovery　and　stabilization　stage　were　investigated　of　surimi　ink　with　AFPs　during　FT　cycles,　and　linking　the　printability,　resolution　and　shape　fidelity,　respectively.　Moreover,　the　evaluation　method　of　3D　printing　quality　during　freeze-thaw　(FT)　cycles　including　printability,　resolution,　and　shape　fidelity　of　surimi　ink　after　FT　was　firstly　established　by　the　line　of　suspension　and　1D　patterns,　2D　patterns,　and　3D　models.　The　results　showed　that　the　rheological　properties　of　ink　were　significantly　improved　due　to　optimum　addition　of　AFPs　during　FT　cycles.　The　yield　stress　(τ0),　consistency　index　(K),　and　shear-thinning　index　(n),　of　2%　AFPs　ink　at　FT　4　times　were　185.93　Pa,　2.506　×　105　Pa•sn,　and　0.062,　respectively,　in　the　extrusion　stage.　In　the　recovery　stage,　the　recovery　time　(T)　and　gelation　time　(Tgel)　were　26.14　s　and　174.83　s.　In　the　stabilization　stage,　the　ink　has　properties　similar　to　solids,　the　interaction　strength　of　molecular　(A)　and　the　number　of　molecular　interactions　(z)　were　1.994　×　103　Pa　s1/z　and　9.488.　It　demonstrated　best　printability,　resolution,　and　shape　fidelity,　even　supporting　23-layer　3D　printing　structures.　This　research　firstly　constructed　a　new　approach　for　the　application　of　AFPs　in　3D　printing　of　frozen　foods.　©　2022　Elsevier　Ltd