Numerous　disposable　plastic　masks　had　been　produced　and　used　for　preventing　the　worldwide　COVID-19　pandemic　effectively.　Discarded　masks　are　a　potential　source　of　microplastic　pollution　in　marine　ecosystems.　The　effect　of　discarded　masks　on　offshore　microorganisms　is　still　unclear.　Herein,　we　profiled　the　interaction　between　the　microplastics　released　by　discarded　masks　and　marine　microbes.　The　effects　of　mask　quantity,　time,　and　environment　on　the　microplastic-related　communities　were　determined.　We　characterized　the　bacterial　communities　of　each　group　using　16S　rRNA　gene　sequencing　and　metagenomic　sequencing　and　correlated　the　community　diversity　to　the　physicochemical　properties　of　seawater.　We　found　that　the　diversity　and　richness　of　microflora　on　the　surface　of　microplastics　with　different　quantity　and　time　varied　significantly.　Proteobacteria　are　the　main　bacteria　on　microplastics,　and　the　KEGG　metabolic　pathway　prediction　shows　that　amino　acid　metabolism　and　carbohydrate　metabolism　were　abundant.　In　addition,　there　was　a　correlation　between　bacterial　communities　and　Antibiotic　Resistance　Ontology　(ARO).　We　used　scanning　electron　microscopy　(SEM)　and　Fourier　transform　infrared　spectroscopy　(FT-IR)　techniques　to　evaluate　the　plastic　polymer　characteristics　of　disposable　medical　masks.　Our　research　shows　that　disposable　medical　masks　immersed　in　seawater　can　alter　the　microbial　community.　This　study　provides　the　most　recent　data　and　insights　into　the　contamination　of　discarded　masks　in　the　marine　environment.