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author:

He, Hongzhang (He, Hongzhang.) [1] | Cheng, Lingjun (Cheng, Lingjun.) [2] | He, Yinghao (He, Yinghao.) [3] | Chen, Jiaming (Chen, Jiaming.) [4] | Song, Liang (Song, Liang.) [5] | Yang, Yuanyuan (Yang, Yuanyuan.) [6] | Zhang, Yun (Zhang, Yun.) [7] | Lin, Zhenyu (Lin, Zhenyu.) [8] (Scholars:林振宇) | Hong, Guolin (Hong, Guolin.) [9]

Indexed by:

EI Scopus SCIE

Abstract:

The lack of large-scale human papillomavirus (HPV) DNA screening is a major contributor to the high incidence and mortality of cervical cancer in economically undeveloped areas. The development of sensitive, rapid, and low-cost screening techniques is urgently needed. Here, DNA nanoflowers encapsulating glucose oxidase and horseradish peroxidase (GHDFs) were synthesized by one-pot rolling circle amplification, and then the GHDFs were used as the cargo of DNA hydrogel and applied for HPV DNA detection. When target DNA was present, the DNA hydrogel cross-linking structure was disrupted, releasing GHDFs, which then catalysed the oxidation of glucose and tetramethylbenzidine in a cascade, generating a significant electrical signal. Signal intensity had a linear relationship with the logarithm of target DNA concentration in the range of 10 fM-1 nM with a detection limit of 3.76 fM. The detection time of the proposed biosensor was 25 min, which was suitable for large-scale HPV DNA screening in economically underdeveloped areas and provides a blueprint for the detection of other DNA of interest.

Keyword:

DNA hydrogel DNA nanoflowers Electrochemical biosensor Enzyme cascade catalytic amplification Human papillomavirus

Community:

  • [ 1 ] [He, Hongzhang]Xiamen Univ, Affiliated Hosp 1, Sch Med, Dept Lab Med, Xiamen 361005, Peoples R China
  • [ 2 ] [Cheng, Lingjun]Xiamen Univ, Affiliated Hosp 1, Sch Med, Dept Lab Med, Xiamen 361005, Peoples R China
  • [ 3 ] [He, Yinghao]Xiamen Univ, Affiliated Hosp 1, Sch Med, Dept Lab Med, Xiamen 361005, Peoples R China
  • [ 4 ] [Chen, Jiaming]Xiamen Univ, Affiliated Hosp 1, Sch Med, Dept Lab Med, Xiamen 361005, Peoples R China
  • [ 5 ] [Yang, Yuanyuan]Xiamen Univ, Affiliated Hosp 1, Sch Med, Dept Lab Med, Xiamen 361005, Peoples R China
  • [ 6 ] [Hong, Guolin]Xiamen Univ, Affiliated Hosp 1, Sch Med, Dept Lab Med, Xiamen 361005, Peoples R China
  • [ 7 ] [Song, Liang]Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China
  • [ 8 ] [Zhang, Yun]Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China
  • [ 9 ] [Song, Liang]Chinese Acad Sci, Xiamen Inst Rare Earth Mat, Haixi Inst, Key Lab Rare Earth Photoelect Funct Mat, Xiamen 361021, Peoples R China
  • [ 10 ] [Zhang, Yun]Chinese Acad Sci, Xiamen Inst Rare Earth Mat, Haixi Inst, Key Lab Rare Earth Photoelect Funct Mat, Xiamen 361021, Peoples R China
  • [ 11 ] [Zhang, Yun]Univ Chinese Acad Sci, Beijing 100049, Peoples R China
  • [ 12 ] [Lin, Zhenyu]Fuzhou Univ, Dept Chem, Minist Educ Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China
  • [ 13 ] [Zhang, Yun]155 Yangqiao Rd West, Fuzhou, Fujian, Peoples R China
  • [ 14 ] [Lin, Zhenyu]Univ Town Fuzhou, 2 Xue Yuan Rd, Fuzhou, Fujian, Peoples R China
  • [ 15 ] [Hong, Guolin]55 Zhenhai Rd, Xiamen, Fujian, Peoples R China

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Source :

SENSORS AND ACTUATORS B-CHEMICAL

ISSN: 0925-4005

Year: 2022

Volume: 371

8 . 4

JCR@2022

8 . 0 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:74

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 8

SCOPUS Cited Count: 8

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

Online/Total:107/10011706
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