Covalent　organic　framework　composites　have　received　great　interest　and　regarded　as　new-generation　porous　materials　in　application　of　sample　preparation.　In　this　work,　an　agaric-like　graphene　oxide　@　covalent　organic　framework　(AGO@COF)　composite　was　first　synthesized　and　used　as　an　ideal　adsorbent　for　enrichment　of　trace　cytokinins　(CKs)　due　to　its　large　specific　surface　area,　rich　in　micro-mesopore　and　organic　functional　groups.　The　contact　areas　between　AGO@COF　composite　and　CKs　were　greatly　increased　due　to　the　special　agaric-like　morphology,　which　would　further　improve　the　extraction　efficiency.　Hence,　a　rapid,　simple　and　efficient　AGO@COF-based　dispersive　solid-phase　extraction　method　for　detecting　four　CKs　(N6-(delta　2-isopentenyl)-adenine,　kinetin,　DL-dihydrozeatin　and　6-benzylaminopurine)　was　successfully　established.　The　proposed　method　had　low　limits　of　detection　(0.1−2.0　pg　mL−1),　good　reproducibility　(RSDs≤3.9%,　n=5)　and　high　recoveries　(84.4−107.7%).　With　this　method,　N6-(delta　2-isopentenyl)-adenine　and　6-benzylaminopurine　in　plants　were　successfully　detected　and　quantified.　Results　showed　that　the　developed　AGO@COF　composite　possessed　superior　extraction　performance　and　potential　application　in　enrichment　of　trace　targets　from　complex　plant　samples.　©　2022