Developing　DNA　nanostructures　as　delivery　vectors　into　organelles　is　a　key　technical　barrier　because　of　their　innate　poor　pharmacokinetic　properties.　Herein,　we　propose　a　unique　concept　for　high　precision　cancer　therapy　by　using　small　gold　nanoparticles　(AuNPs)　as　adhesive　nano-tapes　to　fold　a　long　string　of　aptamer-attached　tetrahedral　DNA　pearls　(Apt-Nano-Tetra)　into　a　spherical　multicenter　DNA　nanostructure　that　can　serve　as　a　nucleus-targeted　drug　delivery　carrier　(Apt-ADMC)　with　a　75～85　times　enhanced　doxorubicin　(Dox)-loading　capacity.　After　specifically　entering　target　tumor　cells,　Dox-Apt-ADMC　is　disassembled　into　individual　AuNPs　surrounded　by　short　Apt-Nano-Tetra,　finally　reaching　cell　nucleus　and　causing　cell　apoptosis.　Even　if　Dox-Apt-ADMC　is　treated　with　fetal　bovine　serum　for　12　h,　the　drug　delivery　ability　is　not　compromised,　contributing　to　high　functional　stability　in　circulation　system.　As　such,　the　systemically-administrated　Dox-Apt-ADMC　shows　7　times　enhanced　tumor　accumulation　and　almost　100%　inhibits　tumor　growth　without　detectable　systemic　toxicity.　©　2022　Elsevier　Ltd