The　formation　of　nacreous　layers　of　abalone　shell　(Aba)　is　a　similar　process　to　the　deposition　of　calcium　salts　in　human　bone,　with　the　main　mechanism　being　the　orderly　mineralization　of　inorganic　matter　mediated　by　organic　bioactive　components.　In　this　study,　3D　printing　technology　was　employed　to　use　the　abundant　calcium　sources　and　bioactive　substances　in　Aba　to　enhance　the　osteoinductive　and　remineralization　capacity　of　materials　in　implants　for　bone　regeneration.　The　novel　hybrid　scaffolds　were　successfully　3D-printed　from　polycaprolactone　(PCL),　which　has　good　toughness　and　processability,　doped　with　powdered　Aba.　When　the　Aba　particle　doping　was　increased　from　0　to　15%,　the　physicochemical　properties　of　PCL　were　virtually　unchanged,　while　the　surfaces　of　scaffolds　became　rough,　and　Aba　particles　were　gradually　exposed.　The　Aba/PCL　scaffolds　had　an　interconnected　pore　structure　with　a　pore　size　of　approximately　200　mu　m　and　over　50%　porosity,　which　was　convenient　for　the　transport　of　nutrients.　With　the　addition　of　Aba,　the　thermodynamic　stability　and　mechanical　properties　of　the　scaffolds　significantly　improved,　and　the　maximum　compressive　strength　and　modulus　reached　1.34　and　1.89　MPa,　respectively,　because　Aba　provided　nucleation　sites　for　nanohydroxyapatite　(nHAP)　to　promote　mineralization.　In　vitro　cell　experiments　showed　that　the　hybrid　scaffolds　had　good　biocompatibility　and　promote　the　proliferation　of　osteoblasts.　In　vivo　results　revealed　that　the　hydroxyapatite　of　organic　matter　and　trace　elements　in　Aba　particles　induced　the　migration　of　stem　cells　and　active　factors　to　the　site　of　a　bone　defect.　The　implantation　of　3D-printed　scaffolds　offered　a　microenvironment　for　osteoblast　attachment　and　proliferation,　which　further　promotes　osteogenesis-related　gene　expression,　such　as　bone　gamma　carboxyglutamate　protein　(BGLAP),　type　I　collagen　(COL1A1),　and　secreted　phosphoprotein　1　(SPP1),　and　facilitated　the　repair　of　a　skull　defect.　This　high-value　applications　of　Aba　have　the　potential　to　improve　environmental　pollution　and　provide　potentially　low-cost,　high-performance　bone　repair　materials　for　clinical　use.