The　one-way　transportation　of　liquids　plays　an　important　role　in　smart　and　wearable　electronics.　Here,　we　report　an　asymmetric　nanofibrous　membrane　(ANM)　with　unidirectional　water　transport　(UWT)　capability　by　integrating　one　superhydrophilic　MXene/Chitosan/Polyurethane　(PU)　nanofiber　membrane　(MCPNM)　and　one　ultrathin　hydrophobic　PU/Polyvinylpyrrolidone　(PVP)　layer　with　a　“bead-on-string”　structure.　The　UWT　performance　shows　long-term　stability　and　can　be　well　maintained　during　the　cyclic　stretching,　abrasion　and　ultrasonic　washing　tests.　The　ANM　exhibits　negative　temperature　coefficient　and　is　served　as　a　temperature　sensor　to　monitor　the　temperature　variation　of　the　environment,　which　can　provide　efficient　alarm　signals　in　a　hot　or　cold　condition.　When　attached　on　person＇s　skin,　the　ANM　displays　a　unique　anti-gravity　UWT　behavior.　The　stretchable,　wearable　and　multi-functional　nanofibrous　composite　membrane　with　an　asymmetric　wettability　shows　potential　applications　in　flexible　and　wearable　electronics,　health　monitoring,　etc.　©　2023　Elsevier　Inc.