Helicobacter　pylori　infection　has　become　a　threat　to　the　world　populations.　This　leads　to　an　urgent　need　of　an　efficient　and　convenient　approach　to　accurately　diagnose　H.　pylori　infection.　Saliva-based　diagnoses　are　particularly　welcomed　for　their　efficiency　and　convenience.　Aiming　at　saliva　sample　analysis,　we　proposed　a　CRISPR/Cas12a-assisted　array,　which　had　integrated　H.　pylori　concentration　detection　and　genotype　screening　functions.　Single-nucleotide　variations　(SNVs)　could　be　distinguished　using　the　screening　array　with　different　probes,　and　an　isothermal　cycling　strategy　was　combined　with　the　trans-cleavage　activity　of　Cas12a　for　signal　amplification　to　improve　accuracy　of　the　diagnosis.　As　a　demonstration,　the　SNV　screening　array　was　fabricated　by　utilizing　the　hybridization　efficiency　difference　caused　by　mismatched　bases.　The　array　was　able　to　successfully　distinguish　between　ten　H.　pylori　genotypes,　and　combined　with　the　successful　SDA　biosensing,　it　had　a　LOD　of　as　low　as　60　fM.　It　was　also　able　to　diagnose　H.　pylori　infection　in　saliva　samples　from　infected　patients.　Together,　the　developed　array　has　a　potential　in　large-scale　clinical　screening　and　is　a　promising　tool　for　the　diagnosis　and　prevention　of　H.　pylori　infection-related　diseases.　©　2022　Elsevier　B.V.