Severe　acute　respiratory　syndrome　coronavirus　(SARS-CoV-2)　is　rampant　all　over　the　world,　and　rapid　and　effective　virus　detection　is　the　best　auxiliary　to　curb　the　spread　of　the　epidemic.　A　diagnosis　can　only　be　made　if　two　or　more　different　nucleic　acid　sequences　are　confirmed　at　the　same　time,　and　in　most　of　traditional　detection　technologies,　these　target　sequences　have　been　detected　separately.　In　this　work,　an　electrochemiluminescent　(ECL)　biosensor　employing　a　single　ECL　probe　as　signal　output　and　responding　to　dual-target　simultaneously　is　proposed　for　the　first　time.　Taking　the　two　sequences　located　in　ORF　1ab　region　and　N　region　of　SARS-CoV-2　gene　sequence　as　the　model　target　and　nitrogen　doped　carbon　quantum　dots　(CDs)　as　ECL　beacon,　supplemented　with　catalytic　hairpin　assembly　(CHA)　reaction　for　signal　amplification,　the　presented　strategy　has　been　successfully　applied　to　the　rapid　detection　of　SARS-CoV-2.　The　developed　SARS-CoV-2　biosensor　based　on　the　series　CHA　systems　can　realize　the　quantitative　determination　of　SARS-CoV-2　in　the　range　of　50　fM　to　200　pM　within　40　min.　Moreover,　the　clinical　validity　of　this　method　has　been　verified　by　the　high　consistency　between　the　detection　results　of　using　this　method　and　those　using　RT-qPCR　for　seven　clinical　pharyngeal　swab　samples.　©　2022　Elsevier　B.V.