This　work　presents　an　integrated　photoelectrochemical,　impedance　and　colorimetric　biosensing　platform　for　flexible　detection　of　cancer　markers　based　on　the　targeted　response　by　combining　liposome　amplification　strategies　and　target-induced　non-in　situ　formation　of　electronic　barriers　as　the　signal　transduction　modality　on　carbon-modified　CdS　photoanodes.　Inspired　by　game　theory,　the　carbon　layer　modified　CdS　hyperbranched　structure　with　low　impedance　and　high　photocurrent　response　was　firstly　obtained　by　surface　modification　of　CdS　nanomaterials.　Through　a　liposome-mediated　enzymatic　reaction　amplification　strategy,　a　large　number　of　organic　electron　barriers　were　formed　by　a　biocatalytic　precipitation　(BCP)　reaction　triggered　by　horseradish　peroxidase　released　from　cleaved　liposomes　after　the　introduction　of　the　target　molecule,　thereby　increasing　the　impedance　characteristics　of　the　photoanode　as　well　as　attenuating　the　photocurrent.　The　BCP　reaction　in　the　microplate　was　accompanied　by　a　significant　color　change,　which　opened　up　a　new　window　for　point-of-care　testing.　Taking　carcinoembryonic　antigen　(CEA)　as　a　proof　of　concept,　the　multi-signal　output　sensing　platform　showed　a　satisfactory　sensitive　response　to　CEA　with　an　optimal　linear　range　of　20　pg　mL−1-100　ng　mL−1.　The　detection　limit　was　as　low　as　8.4　pg　mL−1.　Meanwhile,　with　the　assistance　of　a　portable　smartphone　and　a　miniature　electrochemical　workstation,　the　electrical　signal　obtained　was　synchronized　with　the　colorimetric　signal　to　correct　the　actual　target　concentration　in　the　sample,　further　reducing　the　occurrence　of　false　reports.　Importantly,　this　protocol　provides　a　new　idea　for　the　sensitive　detection　of　cancer　markers　and　the　construction　of　a　multi-signal　output　platform.　©　2023　Elsevier　B.V.