Point-of-care　testing　(POCT)　has　experienced　rapid　development　owing　to　its　advantages　of　rapid　testing,　low　cost　and　strong　operability,　making　it　indispensable　for　analyte　detection　in　outdoor　or　rural　areas.　In　this　study,　we　propose　a　novel　method　for　the　detection　of　aflatoxin　B1　(AFB1)　using　a　dual-signal　readout　approach　within　a　unified　system.　This　method　employs　dual　channel　modes,　namely　visual　fluorescence　and　weight　measurements,　as　the　signal　readouts.　Specifically,　a　pressure-sensitive　material　is　utilized　as　a　visual　fluorescent　agent,　its　signal　can　be　quenched　in　the　presence　of　high　oxygen　pressure.　Additionally,　an　electronic　balance,　commonly　used　for　weight　measurement,　is　adopted　as　another　signal　device,　where　the　signal　is　generated　through　the　catalytic　decomposition　of　H2O2　by　platinum　nanoparticles.　The　experimental　results　demonstrate　that　the　proposed　device　enables　accurate　AFB1　detection　within　the　concentration　range　of　1.5–32　μg　mL−1,　with　a　detection　limit　of　0.47　μg　mL−1.　Moreover,　this　method　has　been　successfully　applied　for　practical　AFB1　detection　with　satisfactory　results.　Notably,　this　study　pioneers　the　use　of　a　pressure-sensitive　material　as　a　visual　signal　in　POCT.　By　addressing　the　limitations　of　single-signal　readout　approaches,　our　method　fulfills　requirements　of　intuitiveness,　sensitivity,　quantitative　analysis　and　reusability.　©　2023