：Glucose　level　is　an　important　evaluation　index　for　the　diagnosis　and　treatment　of　diabetes．In　this　paper，a　novel　portable　glucose　sensor　was　constructed　based　on　laser-induced　graphene/enzyme　elec⁃　trodes.　Micro-flexible　graphene　three-electrode　system（LIG　electrode）was　prepared　by　laser-induced　gra⁃　phene（LIG）technique．The　effect　of　π－π　superposition　occurred　between　the　hydrophobic　terminal　ben-zene　ring　of　1-pyrene　butyric　acid　and　the　six-membered　ring　structure　of　graphene　introduced　the　carboxyl　onto　the　LIG　electrode，then　the　glucose　oxidase（GOx）was　covalently　cross-linked　to　the　surface　of　LIG　for　preparation　of　LIG/GOx　electrodes．　Glucose　sensing　was　carried　out　by　chronoamperometry．　Results　showed　that　the　constructed　sensor　could　be　used　to　detect　glucose　in　the　range　of　5.　0　×　10-5　－1.　0　×　10-2　mol/L　with　a　limit　of　detectton（S/N　=　2）of　5.　0　×　10-5　mol/L．The　method　showed　high　selectivity，sta⁃　bility　and　good　reusability，and　could　be　used　to　detect　glucose　in　serum，urine　and　sweat　samples．LIG　is　a　micro-electrode　material　for　glucose　sensing　with　good　development　prospects，which　is　conducive　to　the　re⁃　alization　of　non-clinical　glucose　detection　and　is　expected　to　be　used　in　the　construction　of　portable　sensors.　©　2023,　China　Association　for　Instrumental　Analysis.　All　rights　reserved.