A　visual　and　quantitative　ELISA-like　method　for　Escherichia　coli　O157:H7　is　developed　based　on　highly　catalytic　and　stable　Au@AuPt　nanoparticles.　The　proposed　enhanced　ELISA　method　can　visually　detect　100　CFU　mL(-1)　O157:H7　with　high　specificity　and　without　the　need　for　strict　low-temperature　reagent　storage,　thereby　increasing　the　utility.　Moreover,　it　is　applicable　to　spiked　tap　water　and　milk　tea　samples　without　additional　treatments.