Molecularly　imprinted　fluorescent　nanoparticles　offer　great　potential　in　highly　selective　and　sensitive　detection　of　small　molecules.　Herein,　a　novel　molecularly　imprinted　fluorescence　nanoprobe　polymer　(CPB-Si-MIP)　was　successfully　synthesized　using　CsPbBr3　perovskite　nanocrystals　(CPB　PNCs)　and　a　silane　coupling　agent　via　the　ligand-assisted　reprecipitation　(LARP)　approach,　sol–gel　method,　and　molecular　imprinting　technology　(MIT).　The　results　demonstrated　a　significant　quenching　of　fluorescence　in　the　CPB-Si-MIP　nanoprobe　with　the　sulfapyridine　(SPD)　template　molecule.　The　linear　range　of　SPD　detection　was　found　to　be　20–100　ng/mL,　with　a　low　detection　limit　of　6.5　ng/mL.　Furthermore,　the　imprinting　factor　(IF)　of　the　nanoprobe　was　2.50,　highlighting　its　high　specificity　and　recognition　ability　toward　SPD　as　the　target　molecule.　©　2023　Elsevier　B.V.