The　sensitive　detection　of　acetylcholinesterase　(AChE)　and　organophosphorus　pesticides　(OPs)　is　very　important　for　the　protection　of　human　health.　Herein,　a　hybrid　material,　Pt　NPs/Fe-MOF,　consisting　of　a　metal-organic　framework　(MIL-88B-NH2,　Fe-MOF)　decorated　with　platinum　nanoparticles　(Pt　NPs),　was　prepared　first　and　exhibited　remarkably　improved　and　excellent　peroxidase-mimicking　activity　compared　to　the　Fe-MOF　material　resulting　from　the　synergistic　catalysis　effect　between　Fe-MOF　and　Pt　NPs,　which　can　effectively　catalyze　3,3′,5,5′-tetramethylbenzidine　(TMB)　oxidation　to　generate　a　blue　product　(oxidized　TMB,　oxTMB).　Interestingly,　in　the　presence　of　AChE　and　acetylcholinesterase,　the　peroxidase-mimicking　activity　from　Pt　NPs/Fe-MOF　was　inhibited　obviously,　and　thus,　a　colorimetric　sensing　platform　for　AChE　can　be　constructed;　more　importantly,　after　the　addition　of　OPs,　this　nanozyme　activity　can　be　recovered,　inducing　the　further　successful　construction　of　a　sensitive　colorimetric　sensing　platform　for　OPs.　The　related　sensing　mechanism　and　condition　optimization　were　studied,　and　the　as-prepared　Pt　NPs/Fe-MOF　nanozyme-based　colorimetric　method　for　AChE　and　OP　detection　displayed　superior　analytical　performances　with　wide　linearities　and　low　detection　limits.　Furthermore,　the　designed　method　offers　satisfactory　real　application　ability.　We　expect　the　as-proposed　Pt　NPs/Fe-MOF　nanozyme-based　colorimetric　sensing　platform　for　AChE　and　OPs　via　the　enzyme　cascade　reaction　to　show　great　potential　application.　©　2023　American　Chemical　Society