Cellulose-based　extraction　material　(Cell-COOH)　was　prepared　from　cellulose　modification　with　acid　anhydride　for　amphetamine　enrichment　in　urine,　and　characterized　by　FTIR,　XRD,　SEM　and　XPS.　A　solid-phase　extraction　technique　coupled　with　high　performance　liquid　chromatography　based　on　Cell-COOH　was　established　for　the　determination　of　amphetamine　in　urine.　The　solid-phase　extraction　conditions　were　then　optimized,　while　the　interference　resistance　and　reusability　of　Cell-COOH　were　evaluated　and　the　feasibility　of　this　technique　was　verified.　The　results　showed　that　the　surface　of　Cell-COOH　contained　carboxyl　functional　group　with　good　adsorption　to　amphetamine.　The　method　showed　good　specificity　and　anti-interference　ability　under　the　interference　of　other　abused　drugs　and　urine　co-existing　ions,　and　the　extraction　efficiency　could　remain　above　92%　when　used　for　the　seventh　time.　Under　the　optimal　extraction　conditions　of　pH=8,　extractant　dosage　0.50　g/L,　extraction　time　30　min,　eluent　of　0.8%　(volume　fraction)　hydrochloric　acid,　eluent　dosage　0.08　L/g,　elution　time　20　min,　the　linear　range　of　the　method　was　among　0.01~2.00　mg/L　(R2=0.9973),　and　the　limit　of　detection　was　2.5　μg/L　with　the　quantification　limit　of　8.0　μg/L.　The　extraction　recovery　of　amphetamine　could　reach　74.41%~82.14%　when　using　Cell-COOH　as　solid　phase　extractant　in　actual　urine,　with　the　relative　deviation　(n=3)　of　2.32%~6.85%.　©　2023　Fine　Chemicals.　All　rights　reserved.