Imitating　catalytic　sites　of　natural　enzymes　is　a　promising　approach　to　accurately　improve　the　catalytic　performances　of　nanozymes.　Herein,　inspired　by　the　structure　of　natural　horseradish　peroxidase　(HRP),　three-component　condensation　strategy　is　unitized　to　design　histidine　(His)　functionalized　iron　porphyrin　covalent　organic　frameworks　(Fe-COF-H3)　nanozyme　with　peroxidase-like　(POD-like)　activity.　The　introduced　His　could　form　favorable　microenvironment　around　the　iron　porphyrin　structure,　enhancing　the　catalytic　capacity　of　COF　nanozyme.　Taking　advantage　of　its　excellent　POD-like　activity,　Fe-COF-H3　was　utilized　as　a　carrier　to　immobilize　urate　oxidase　(UOx)　for　the　construction　of　enzyme-nanozyme　cascade　system　(UOx@Fe-COF-H3).　The　UOx@Fe-COF-H3　was　successfully　applied　for　the　colorimetric　detection　of　uric　acid　(UA)　in　biological　samples.　This　work　not　only　offers　a　new　insight　to　develop　high-efficiency　COF　nanozymes,　but　also　expands　the　application　of　COF　nanozyme　for　the　construction　of　enzyme-nanozyme　cascade　system.　©　2023　Elsevier　B.V.