Hydrogen　sulfide　(H2S)　is　an　important　sulfur-containing　signaling　molecule　in　the　human　body,　as　well　as　a　highly　toxic　gas　that　is　soluble　in　water.　A　variety　of　pathological　processes　are　associated　with　abnormal　metabolism　of　hydrogen　sulfide,　so　it　is　very　important　to　develop　an　assay　that　can　rapidly　and　specifically　detect　H2S.　In　this　work,　two　iridium(Ⅲ)　complexes　were　designed　and　synthesized　using　ester　groups　as　reaction　sites　for　recognition　of　H2S.　As　the　H2S　concentration　increased,　the　photoluminescence　of　the　probe　was　rapidly　quenched　within　5　s,　with　a　detection　limit　as　low　as　0.45　μM.　Among　them,　the　probe　Ir1,　whose　luminescence　was　located　in　the　visible　region,　had　been　successfully　used　for　the　detection　of　trace　amounts　of　H2S　in　water　samples　with　a　recovery　rate　of　92.5　∼　104.0%　and　a　relative　standard　deviation　(RSD,　n　=　5)　of　＜　2.8%.　The　luminescence　of　probe　Ir2　was　located　in　the　near-infrared　region,　which　could　avoid　the　interference　of　biological　autofluorescence　and　be　successfully　used　for　luminescence　imaging　of　H2S　in　HeLa　cells.　This　study　provided　a　promising　method　for　rapid　detection　of　trace　H2S　in　water　samples　and　cellular　microenvironments.　©　2023　Elsevier　B.V.