The　combined　contamination　of　multiple　mycotoxins　(MTs)　is　a　matter　of　utmost　concern,　posing　significant　threats　to　both　the　economy　stability　and　the　well-being　of　humans　and　animals.　Consequently,　there　is　an　urgent　demand　for　highly　sensitive　and　portable　multiplex　detection　methods　to　effectively　identify　and　quantify　MTs.　The　primary　objective　of　this　paper　is　to　provide　a　comprehensive　overview　of　the　methods　to　detect　multiple　MTs　in　agricultural　products,　including　instrumental　and　biosensor　approaches　published　in　the　last　five　years.　Specifically,　we　emphasize　detection　methods　for　antibody　(ab)-　and　aptamer　(apt)-based　biosensors,　while　undertaking　a　comparative　analysis　of　their　performance,　particularly　focusing　on　the　sensitivity.　Furthermore,　this　review　proposes　several　promising　technologies　that　can　be　leveraged　in　the　future　and　outlines　the　challenges　and　prospects　associated　with　achieving　rapid,　accurate,　and　intelligent　detection　of　MTs.　©　2023　Elsevier　Ltd