Novel　mechanical　metamaterial　structures　usually　consist　of　a　number　of　simple　cells　combined　by　three-dimensional　regular　arrays.　Due　to　their　extraordinary　physical　properties,　these　mechanical　metamaterial　structures　often　have　the　advantages　of　light　weight,　high　strength,　multi-stability,　excellent　energy　absorption　capability,　etc.,　and　are　becoming　a　research　focus　in　many　fields.　In　order　to　further　enrich　metamaterial　structures,　more　high-quality　cellular　structures　need　to　be　explored.　Origami　structure　has　the　advantages　of　simple　structure　and　easy　folding,　which　provides　an　important　way　to　construct　metamaterial　cells.　In　this　paper,　a　twofold-symmetric　kirigami　(Twofold-sym　Kiri)　pattern　with　good　symmetry　is　adopted,　and　a　cell　with　negative　Poisson＇s　ratio　and　single　degree　of　freedom　folding　is　proposed.　By　analyzing　the　influence　of　the　geometric　parameters　of　the　cell　structure　on　the　folding　motion,　it　is　found　that　the　cell　has　a　stretching　characteristic,　namely　negative　Poisson＇s　ratio.　When　the　variable　γ　of　the　kirigami　pattern　approaches　0,　the　cell　tends　to　the　classical　reentrant　honeycomb　structure.　Compared　to　the　classical　reentrant　honeycomb　structure,　the　proposed　cell　has　the　advantage　of　single　DOF　which　will　benefit　to　construct　large　deployable　energy-absorption　structures　quickly.　With　the　usage　of　the　self-locking　function　caused　by　the　physical　interference　during　folding,　the　cellular　structure　in　the　folding　limit　state　has　the　load　capacity.　Through　the　static　compression　test,　it　is　found　that　the　Twofold-sym　Kiri　structure　has　better　load　capacity　than　the　reentrant　honeycomb　structure,　which　is　an　excellent　origami　metamaterial　structure.　The　proposed　cell　provides　the　possibility　for　exploring　more　new　metamaterial　structures　and　lays　the　theoretical　analysis　foundation　for　it.　©　2023　Editorial　Office　of　Chinese　Journal　of　Mechanical　Engineering.　All　rights　reserved.