Driven　by　sustainable　development　and　low-carbon　policies,　many　high-performance　bio-based　polyesters　are　produced.　Our　previous　work　uncovered　the　high　barrier　but　low　thermal　and　mechanical　properties　of　a　renewable　2,6-pyridinedicarboxylic-based　polyester.　In　this　article,　the　copolymerization　with　isosorbide　as　a　second　diol　is　considered　to　ameliorate　these　properties.　The　chemical　structures　of　copolyesters　and　the　successful　introduction　of　isosorbide　were　confirmed　by　FT-IR　and　1H　NMR.　The　1H　NMR　spectra　also　allowed　the　calculation　of　isosorbide　ratio　in　polymers.　GPC　revealed　a　decrease　in　molar　masses　with　the　increase　of　isosorbide　ratio.　Contact　angle　and　water/gas　permeation　measurements　disclosed　an　increase　in　hydrophilicity　and　water/gas　permeability　with　the　introduction　of　isosorbide.　TGA　and　DSC　analyses　demonstrated　an　enhancement　in　thermal　stability:　8　mol%　of　isosorbide　to　the　total　diol　ratio　increased　the　5　%　weight　loss　temperature　(T5%)　by　9　degrees　C,　the　maximum　weight　loss　temperature　(Tmax)　by　8　degrees　C　and　the　glass　transition　temperature　(Tg)　by　6　degrees　C.　DSC　investigation　showed　that　the　incorporation　of　isosorbide　significantly　influenced　the　crystallization　properties,　particularly　from　the　melt,　by　decreasing　the　crystallinity.　XRD　patterns　indicated　the　presence　of　similar　crystallization　units　in　these　polyesters.　Tensile　test　revealed　that　incorporating　8　mol%　of　isosorbide　greatly　improved　the　mechanical　properties　by　doubling　Young＇s　modulus　and　tripling　the　strength　at　break.　Such　enhancement　in　thermal　and　mechanical　properties　is　important　for　high　gas　barrier　food　packaging　applications　of　these　polyesters.