Bacterial　biofilm　infection　is　a　serious　obstacle　to　clinical　therapeutics.　Photodynamic　therapy　(PDT)　plays　a　dynamic　role　in　combating　biofilm　infection　by　utilizing　reactive　oxygen　species　(ROS)-induced　bacterial　oxidation　injury,　showing　advantages　of　mild　side　effects,　spatiotemporal　controllability　and　little　drug　resistance.　However,　superfluous　glutathione　(GSH)　present　in　biofilm　and　bacteria　corporately　reduces　ROS　levels　and　seriously　affects　PDT　efficiency.　Herein,　we　have　constructed　a　Cu2+-infused　porphyrin　metal-organic　framework　(MOF@Cu2+)　for　the　enhanced　photodynamic　combating　of　biofilm　infection　by　the　maximum　depletion　of　GSH.　Our　results　show　that　the　released　Cu2+　from　porphyrin　MOF@Cu2+　could　not　only　oxidize　GSH　in　biofilm　but　also　consume　GSH　leaked　from　ROS-destroyed　bacteria,　thus　greatly　weakening　the　antioxidant　system　in　biofilm　and　bacteria　and　dramatically　improving　the　ROS　levels.　As　expected,　our　dual-enhanced　PDT　nanoplatform　exhibits　a　strong　biofilm　eradication　ability　both　in　vitro　and　in　an　in　vivo　biofilm-infected　mouse　model.　In　addition,　Cu2+　can　promote　biofilm-infected　wound　closing　by　provoking　cell　immigration,　collagen　sediment　and　angiogenesis.　Besides,　no　apparent　toxicity　was　detected　after　treatment　with　MOF@Cu2+.　Overall,　our　design　offers　a　new　paradigm　for　photodynamic　combating　biofilm　infection.　©　2024　The　Royal　Society　of　Chemistry.