In　this　work,　a　portable　electrochemical　glucose　sensor　was　studied　based　on　a　laser-induced　graphene　(LIG)　composite　electrode.　A　flexible　graphene　electrode　was　prepared　using　LIG　technology.　Poly(3,4-ethylene　dioxythiophene)　(PEDOT)　and　gold　nanoparticles　(Au　NPs)　were　deposited　on　the　electrode　surface　by　potentiostatic　deposition　to　obtain　a　composite　electrode　with　good　conductivity　and　stability.　Glucose　oxidase　(GOx)　was　then　immobilized　using　glutaraldehyde　(GA)　to　create　an　LIG/PEDOT/Au/GOx　micro-sensing　interface.　The　concentration　of　glucose　solution　is　directly　related　to　the　current　value　by　chronoamperometry.　Results　show　that　the　sensor　based　on　the　LIG/PEDOT/Au/GOx　flexible　electrode　can　detect　glucose　solutions　within　a　concentration　range　of　0.5　×　10−5　to　2.5　×　10−3　mol　L−1.　The　modified　LIG　electrode　provides　the　resulting　glucose　sensor　with　an　excellent　sensitivity　of　341.67　μA　mM−1　cm−2　and　an　ultra-low　limit　of　detection　(S/N　=　3)　of　0.2　×　10−5　mol　L−1.　The　prepared　sensor　exhibits　high　sensitivity,　stability,　and　selectivity,　making　it　suitable　for　analyzing　biological　fluid　samples.　The　composite　electrode　is　user-friendly,　and　can　be　built　into　a　portable　biosensor　device　through　smartphone　detection.　Thus,　the　developed　sensor　has　the　potential　to　be　applied　in　point-of-care　platforms　such　as　environmental　monitoring,　public　health,　and　food　safety.　©　2024　The　Royal　Society　of　Chemistry