The　specificity，programmability　and　biocompatibility　of　DNA　materials　make　them　advantageous　for　applications　in　bio-detection　and　drug　delivery.　To　further　expand　the　applications　and　synthesis　methods　of　DNA　materials，in　this　study，Zn2+/DNA　self-assembled　nanoparticles（ZDNPs）with　tumor-responsive　marker　microRNA-21　(miR-21)　are　simply　and　efficiently　synthesized　by　utilizing　the　cooperative　interaction　between　DNA　and　metal　ions.　Through　the　specific　complementarity　of　the　DNA　hairpin　structure　in　ZDNPs　with　miR-21，the　fluorescent　signal　is　activated　and　Zn2+　is　released，which　result　in　intracellular　ROS　production　and　can　be　used　for　fluorescence　imaging　and　oxidative　stress　therapy　of　tumor.　The　synthesized　ZDNPs　are　uniformly　shaped　spherical　particles　that　can　effectively　load　Zn2+.　Depending　on　the　responsive　experiment　of　ZDNPs　to　miR-21，it　is　verified　that　there　is　a　good　linear　fluorescence　response　signal　with　specificity　between　ZDNPs　and　miR-21　concentration.　The　linear　response　range　is　5~160　nmol/L，and　the　detection　limit　is　5　nmol/L.　The　intracellular　effects　of　ZDNPs　are　also　investigated，and　the　results　show　that　they　can　perform　responsive　fluorescence　imaging，and　mediate　apoptosis　of　tumor　cells　through　the　oxidative　stress　pathway.　As　for　in　vivo　fluorescence　imaging　and　tumor　therapy，ZDNPs　demonstrate　specific　and　sustained　tracing　ability　at　tumor　lesion　sites　and　produce　significant　growth　inhibition　effects　on　tumor.　©　2024　The　Author(s).