T-2　toxin　is　widely　found　in　nature　and　also　contaminates　field　crops　and　grain　storage,　posing　a　potential　threat　to　human　health.　In　this　study,　an　ultra-sensitive　electrochemiluminescence　(ECL)　aptasensor　has　been　developed　for　the　detection　of　T-2　toxin　using　Ti3C2　MXene-based　nanomaterials　functionalized　with　gold　nanorods　(AuNRs)　and　Ru(bpy)32+　(Ti3C2@AuNRs-Ru)　as　the　luminescent　indicator.　Specifically,　gold　nanorods　are　grown　in　situ　on　the　surface　of　Ti3C2　MXene,　and　Ru(bpy)32+　is　loaded　onto　Ti3C2@AuNRs　via　electrostatic　force　to　form　the　Ti3C2@AuNRs-Ru　luminescent　probe,　which　is　then　modified　on　the　electrode　surface.　The　aptamer　of　T-2　toxin　is　connected　to　the　electrode　surface　through　Au-S　bonds,　and　then　the　capture　chain　(cDNA)　with　a　quenching　probe　of　ferrocene　is　introduced　to　the　system.　Ferrocene　can　quench　the　luminescence　of　the　probe,　reducing　the　ECL　signal.　In　the　presence　of　T-2　toxin,　it　binds　to　the　aptamer　with　higher　affinity,　so　the　quenching　probe　is　competed　down,　resulting　in　the　recovery　of　the　ECL　signal.　The　increase　in　ECL　signal　is　linearly　correlated　with　the　logarithm　of　T-2　toxin　concentration,　with　the　detection　range　of　50　fg/mL　to　50　ng/mL　and　a　detection　limit　of　6.44　fg/mL.　This　strategy　utilizes　the　high　catalytic　activity　and　excellent　conductivity　of　MXene,　improving　the　sensitivity　significantly.　The　proposed　sensor　has　been　successfully　applied　for　the　detection　of　actual　samples　and　provides　a　new　strategy　for　the　detection　of　hazardous　pollutants.　©　2023