Conductive　hydrogels　as　promising　candidates　of　wearable　electronic　have　attracted　considerable　interest　in　health　monitoring,　multifunctional　electronic　skins,　and　human-machine　interfaces.　However,　to　simultaneously　achieve　excellent　electrical　properties,　long-term　stability,　and　water　vapor　permeability　of　conductive　hydrogels　remains　a　challenge.　Herein,　a　highly　conductive　hydrogel　(MPRS)　with　high　breathability　(5.90　mg∙cm−2∙h−1),　excellent　electrical　conductivity　(6.67　S/m),　high　elongation　(315%),　and　remarkable　water　retention　properties　was　prepared　adopting　cold　freeze-thawing　method.　MXene,　poly　(3,　4-ethylenedioxythiophene):　poly　(styrenesulfonate)　(PEDOT:　PSS),　and　reduced　graphene　oxide　were　used　as　conductive　materials,　while　silk　fibroin　(SF)　and　polyvinyl　alcohol　(PVA)　were　used　as　the　gel　matrix.　Lithium　chloride　solution　was　used　as　a　solvent　during　the　preparation　process.　The　strain　sensor　based　on　this　hydrogel　demonstrates　rapid　response　(0.33　s)　and　recovery　ability　(0.31　s)　to　strain　stimuli.　Upon　250　cycles　of　stretching　at　35%　strain,　the　output　signal　remains　stable　and　highly　repeatable,　ensuring　accurate　detection　of　human　joint　movements　and　facial　expressions.　The　fabrication　of　flexible　electrodes　using　the　hydrogel　enabled　real-time　monitoring　of　human　electromyography　and　electrocardiogram　signals.　The　applications　in　a　2D　sensor　array　and　human-machine　interaction　interfaces　also　proved　the　excellent　performance　of　this　conductive　hydrogels　in　wearable　sensor.　©　2024　Elsevier　Ltd