An　intelligent　sensor　of　ascorbic　acid　（AA）based　on　graphene　microelectrode　was　constructed.　By　designing　the　pattern　of　a　three-electrode　system　which　is　compatible　with　the　U-disk　electrochemical　analyzer，a　portable　intelligent　detection　method　for　AA　was　developed　based　on　graphene　microelectrode　using　laser　direct　writing　technology.　The　electrochemical　response　of　the　prepared　graphene　electrode　to　AA　was　assessed　by　cyclic　voltammetry　through　smart　phone　detection.　The　results　showed　that　the　concentration　of　AA　had　a　good　linear　relationship　in　the　range　of　0.01-0.5　mg/mL　at　pH　1.　The　designed　graphene　microelectrode　is　compatible　with　the　PalmSens　U-disk　electrochemical　analyzer.　The　prepared　method　can　be　used　for　the　detection　of　sweat　and　urine　samples.　©　2023,　Youke　Publishing　Co.,Ltd.　All　rights　reserved.