Digital　microfluidic,　as　an　emerging　and　potential　technology,　diversifies　the　biochemical　applications　platform,　such　as　protein　dilution　sewage　detection.　At　present,　a　vast　majority　of　universal　cyberphysical　digital　microfluidic　biochips　(DMFBs)　transmit　data　through　wires　via　personal　computers　and　microcontrollers　(like　Arduino),　consequently,　susceptible　to　various　security　threats　and　with　the　popularity　of　wireless　devices,　losing　competitiveness　gradually.　On　the　premise　that　security　be　ensured　first　and　foremost,　calls　for　wireless　portable,　safe,　and　economical　DMFBs　are　imperative　to　expand　their　application　fields,　engage　more　users,　and　cater　to　the　trend　of　future　wireless　communication.　To　this　end,　a　new　cyber-physical　DMFB　called　PortableLab　is　proposed　in　this　paper,　which　guarantees　data　security　through　wireless　sensors　at　low　cost.　After　considering　the　security,　computing　consumption,　and　cost,　a　mobile　module　is　added.　In　addition,　the　improved　Advanced　Encryption　Standard　(AES)　and　Cyclic　Redundancy　Check　(CRC)　algorithms　are　utilized　to　ensure　the　integrity　and　confidentiality　of　data　transmission.　Ultimately,　all　the　security　analysis,　cost　analysis,　and　experimental　results　on　multiple　protocols　demonstrate　the　feasibility　of　the　proposed　PortableLab　DMFB　in　time　and　space.　©　2013　IEEE.