Colorectal　cancer　is　a　prevalent　malignancy　globally,　often　linked　to　chronic　colitis.　Terahertz　technology,　with　its　noninvasive　and　fingerprint　spectroscopic　properties,　holds　promise　in　disease　diagnosis.　This　study　aimed　to　explore　terahertz　technology＇s　application　in　colitis-associated　cancer　using　a　mouse　model.　Mouse　colorectal　tissues　were　transformed　into　paraffin-embedded　blocks　for　histopathological　analysis　using　HE　staining.　Terahertz　transmission　spectroscopy　was　performed　on　the　tissue　blocks.　By　comparing　terahertz　absorption　differences,　specific　frequency　bands　were　identified　as　optimal　for　distinguishing　cancerous　and　normal　tissues.　The　study　revealed　that　terahertz　spectroscopy　effectively　differentiates　colitis-related　cancers　from　normal　tissues.　Remarkably,　1.8　THz　emerged　as　a　potential　optimal　frequency　for　diagnosing　colorectal　cancer　in　mice.　This　suggests　the　potential　for　rapid　histopathological　diagnosis　of　colorectal　cancer　using　terahertz　technology.　©　2023　Wiley-VCH　GmbH.