The　control　of　nanoparticle　morphology　can　effectively　change　their　properties.　Adjusting　the　aggregation　state　of　bovine　serum　albumin-coated　gold　nanoclusters　(BSA-Au　NCs)　can　regulate　the　enzyme-mimicking　catalytic　activity.　Hyaluronic　acid　(HA)　induces　the　aggregation　of　BSA-Au　NCs,　leading　to　the　shielding　of　the　catalytic　active　sites　and　a　decrease　in　activity;　this　feature　has　been　utilized　to　design　a　multicolor　biosensor　for　hyaluronidase　(HAase).　The　presence　of　HAase　can　hydrolyze　the　glycosidic　bonds　in　HA,　causing　the　aggregated　BSA-Au　NCs　to　disperse　and　express　their　catalytic　activity,　which　in　turn　catalyzes　the　etching　of　Au　nanobipyramids　(Au-NBPs)　in　the　presence　of　3,3′,5,5′-tetramethylbenzidine　(TMB),　resulting　in　a　morphological　transition　from　bipyramidal　to　ellipsoidal　and　spherical　shapes,　while　the　solution　displays　a　variety　of　colors.　Visual　recognition　of　these　multicolor　changes　establishes　a　relationship　between　the　enzyme　activity　and　color.　Based　on　the　controlled　aggregation　and　dispersion　of　BSA-Au　NCs　and　the　etching　of　Au-NBPs,　a　simple　multicolor　HAase　biosensor　was　designed.　The　proposed　biosensor　shows　a　linear　response　to　HAase　concentrations　in　the　range　of　5-80　U/mL,　with　clear　color　changes　under　optimized　conditions.　The　limit　of　detection　(LOD)　of　the　sensor　was　determined　to　be　1.98　U/mL　(LOD　=　3s/k).　This　method　successfully　applies　the　changing　of　enzyme-mimicking　catalytic　activity　nanoparticles　for　colorimetric　analysis　detection.　©　2024　American　Chemical　Society.