In　the　minerals　processing　industry,　the　surface　chemistry　of　mineral　particles　and　its　real-time　detection　can　significantly　enhance　process　performance,　and　ultimately　leading　to　automotive　and　intelligent　control.　The　adsorption　of　collector　molecule　onto　bulk　mineral　specimens　could　be　investigated　with　the　help　of　shellisolated　nanoparticle　enhanced　Raman　spectroscopy　(SHINERS).　However,　this　method　is　unsuitable　for　the　online　detection　of　particles　fluid　consisted　of　micro-sized　chalcocite　that　encountered　in　industrial　production　processes.　In　this　work,　a　novel　strategy　of　shell-isolated　nanoparticles　synthesis　by　electrodeposition　of　gold　nanoparticles　film　and　isolation　of　this　film　with　crosslinked　silica　monolayer　was　proposed.　The　adsorption　of　2mercaptobenzothiazole　(MBT),　a　typical　flotation　collector,　onto　a　copper　sulfide　mineral,　chalcocite　was　measured　in-situ　with　the　help　of　such　a　SERS　substrate.　Enhancement　factors　of　106-107　was　calculated　based　on　an　idealized　model.　Furthermore,　we　discussed　the　stability　of　the　silica　isolation　monolayer　under　high-power　laser　irradiation.