The　use　of　two-dimensional　(2D)　layered　metal-organic　frameworks　(MOFs)　as　self-sacrificial　templates　has　been　proven　to　be　a　successful　method　to　create　high-efficiency　Selenium　(Se)-containing　electrocatalysts　for　overall　water　splitting.　Herein,　two　strategies　are　then　utilized　to　introduce　Se　element　into　the　Co-Fe　MOF,　one　being　the　etching　of　as-prepared　MOF　by　SeO2　solution,　and　the　other,　the　replacing　of　SCN-　with　SeCN-　as　the　construction　unit.　The　electrochemical　activity　of　the　pristine　2D　MOF　and　their　calcinated　derivatives　for　catalyzing　the　hydrogen　evolution　reaction　(HER)　and　oxygen　evolution　reaction　(OER)　is　evaluated　and　further　discussed.　It　is　found　that　the　effect　of　introducing　Se　on　improving　electrochemical　catalytic　activity　is　significant　for　the　HER　process.　Specifically,　the　calcinated　derivative　in　the　replacing　method　exhibits　an　overpotential　of　235　mV　for　HER　and　270　mV　for　OER　at　a　current　density　of　10　mA/cm2.　For　comparing　the　two　methods　of　introducing　Se　element　into　MOF,　similar　electrocatalytic　activity　can　be　achieved　on　the　their　calcinated　derivatives.　The　high　electrochemical　performance　of　2D　CoFe-MOF　derivatives　may　be　resulted　from　the　unique　2D　hierarchical　porous　structure　and　strong　synergistic　effect　between　different　components　in　the　material.