Uncovering the Nonnegligible Mechanism of Degrading Structural Extracellular Polymeric Substances for Nitrate Removal in Psychrophilic (15 °C) Waste-Activated Sludge Fermentation - Details

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Wastewater　treatment　plants　(WWTPs)　are　being　challenged　to　operate　as　carbon-neutral　and　energy-efficient　systems.　The　structural　extracellular　polymeric　substances　(St-EPS)　in　waste-activated　sludge　(WAS),　such　as　alginate,　were　revealed　to　form　an　architectural　gel　matrix,　hindering　the　psychrophilic　hydrolysis　and　acidogenesis　and　its　application　in　nitrate　removal.　Consequently,　volatile　fatty　acid　(VFAs)　production　for　nitrate　removal　at　15　°C　is　demonstrated　by　a　novel,　enriched　psychrophilic　consortium　for　alginate-degrading　and　nitrate-removing.　Both　alginate　lyase　(EC　4.2.2.3)　and　oligo-alginate　lyase　(EC　4.2.2.26)　are　identified　for　alginate　utilization　by　the　metaproteomics　analysis.　A　high　nitrate　removal　rate　of　7.6　(WAS　as　the　substrate)　to　13.0　(alginate　as　the　substrate)　mgN/(gVSS　h)　was　achieved.　By　combining　high-throughput　and　PacBio　sequencing　and　metagenomics　analysis,　two　genera　of　Bacteroides　(69.4%)　and　Dysgonomons　(12.8%)　are　revealed　to　enhance　psychrophilic　WAS　fermentation.　The　VFAs　can　be　utilized　by　denitrifiers　for　nitrate　removal　in　an　indirect　pathway.　Moreover,　other　genera　of　denitrifiers　(Pseudomonas,　0.11%;　and　Massilla,　0.003%)　can　excrete　alginate　lyase,　indicating　the　dual　merits　of　St-EPS　degradation　and　nitrate　removal　in　the　direct　pathway.　This　work　extends　the　application　of　WAS　for　denitrifiers,　providing　a　new　carbon-neutral　and　energy-efficient　system　in　WWTPs,　especially　under　psychrophilic　conditions.　©　2024　American　Chemical　Society.

Keyword：

metagenomics metaproteomics nitrate removal psychrophilic consortium of alginate-degrading and nitrate-removing structural extracellular polymeric substances waste-activated sludge

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[ 1 ] [Wang Y.-B.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 2 ] [Tang J.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 3 ] [Yan Y.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 4 ] [Hong S.-D.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 5 ] [Zheng S.-J.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 6 ] [Huang X.-C.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 7 ] [Chen M.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 8 ] [Fu S.-F.]Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China
[ 9 ] [Chen X.]College of Environment and Safety Engineering, Fuzhou University, Fuzhou, 350116, China
[ 10 ] [Zeng R.J.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 11 ] [Dai K.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China
[ 12 ] [Zhang F.]Center of Wastewater Resource Recovery, College of Resources and Environment, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, China

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Uncovering the Nonnegligible Mechanism of Degrading Structural Extracellular Polymeric Substances for Nitrate Removal in Psychrophilic (15 degree celsius) Waste-Activated Sludge Fermentation
2024，ACS ES&T WATER

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Source ：

ACS ES and T Water

ISSN： 2690-0637

Year： 2024

Issue： 5

Volume： 4

Page： 2270-2280

CAS Journal Grade：3

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SCOPUS Cited Count： 1

ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 3

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